rabbit anti human rtp801 Search Results


94
Proteintech rabbit polyclonal anti ddit4
Rabbit Polyclonal Anti Ddit4, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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novus biologicals nbp1-77321ss

Nbp1 77321ss, supplied by novus biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech rabbit polyclonal redd1 antibody
Figure 1. Effect of <t>REDD1</t> on tumor cell growth in vitro. (A) REDD1 expression was detected by Western blotting in T80, T80K, T80H, T29, T29K and T29H cell lines. (B) Western blot analysis showed increased REDD1 expression after introduction of REDD1 cDNA into immortalized ovarian epithelial cell lines T80 and T29 and decreased REDD1 expression after REDD1 siRNA knockdown in RAS-transformed ovarian epithelial T29H cells. (C and D) T80-REDD1 and T29-REDD1 cells displayed statistically significant increases in cell proliferation and colony formation compared with findings in parental T80 and T29 cells, whereas cells expressing REDD1 siRNA showed reduced cell proliferation and colony formation compared with parental cells infected with scrambled siRNA. (C) Cell proliferation rate following overexpression of REDD1 or siRNA knockdown for all three groups. (D) Number of colonies of anchorage-independent cell growth on soft agar in the presence of REDD1 overexpression or knockdown. (p < 0.05).
Rabbit Polyclonal Redd1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck KGaA polyclonal antibodies against full length rtp801
Figure 1. Effect of <t>REDD1</t> on tumor cell growth in vitro. (A) REDD1 expression was detected by Western blotting in T80, T80K, T80H, T29, T29K and T29H cell lines. (B) Western blot analysis showed increased REDD1 expression after introduction of REDD1 cDNA into immortalized ovarian epithelial cell lines T80 and T29 and decreased REDD1 expression after REDD1 siRNA knockdown in RAS-transformed ovarian epithelial T29H cells. (C and D) T80-REDD1 and T29-REDD1 cells displayed statistically significant increases in cell proliferation and colony formation compared with findings in parental T80 and T29 cells, whereas cells expressing REDD1 siRNA showed reduced cell proliferation and colony formation compared with parental cells infected with scrambled siRNA. (C) Cell proliferation rate following overexpression of REDD1 or siRNA knockdown for all three groups. (D) Number of colonies of anchorage-independent cell growth on soft agar in the presence of REDD1 overexpression or knockdown. (p < 0.05).
Polyclonal Antibodies Against Full Length Rtp801, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio anti ddit4
Figure 1. Effect of <t>REDD1</t> on tumor cell growth in vitro. (A) REDD1 expression was detected by Western blotting in T80, T80K, T80H, T29, T29K and T29H cell lines. (B) Western blot analysis showed increased REDD1 expression after introduction of REDD1 cDNA into immortalized ovarian epithelial cell lines T80 and T29 and decreased REDD1 expression after REDD1 siRNA knockdown in RAS-transformed ovarian epithelial T29H cells. (C and D) T80-REDD1 and T29-REDD1 cells displayed statistically significant increases in cell proliferation and colony formation compared with findings in parental T80 and T29 cells, whereas cells expressing REDD1 siRNA showed reduced cell proliferation and colony formation compared with parental cells infected with scrambled siRNA. (C) Cell proliferation rate following overexpression of REDD1 or siRNA knockdown for all three groups. (D) Number of colonies of anchorage-independent cell growth on soft agar in the presence of REDD1 overexpression or knockdown. (p < 0.05).
Anti Ddit4, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc s6 ribosomal protein
Figure 1. Effect of <t>REDD1</t> on tumor cell growth in vitro. (A) REDD1 expression was detected by Western blotting in T80, T80K, T80H, T29, T29K and T29H cell lines. (B) Western blot analysis showed increased REDD1 expression after introduction of REDD1 cDNA into immortalized ovarian epithelial cell lines T80 and T29 and decreased REDD1 expression after REDD1 siRNA knockdown in RAS-transformed ovarian epithelial T29H cells. (C and D) T80-REDD1 and T29-REDD1 cells displayed statistically significant increases in cell proliferation and colony formation compared with findings in parental T80 and T29 cells, whereas cells expressing REDD1 siRNA showed reduced cell proliferation and colony formation compared with parental cells infected with scrambled siRNA. (C) Cell proliferation rate following overexpression of REDD1 or siRNA knockdown for all three groups. (D) Number of colonies of anchorage-independent cell growth on soft agar in the presence of REDD1 overexpression or knockdown. (p < 0.05).
S6 Ribosomal Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Epitomics corp phospho)-src
Figure 1. Effect of <t>REDD1</t> on tumor cell growth in vitro. (A) REDD1 expression was detected by Western blotting in T80, T80K, T80H, T29, T29K and T29H cell lines. (B) Western blot analysis showed increased REDD1 expression after introduction of REDD1 cDNA into immortalized ovarian epithelial cell lines T80 and T29 and decreased REDD1 expression after REDD1 siRNA knockdown in RAS-transformed ovarian epithelial T29H cells. (C and D) T80-REDD1 and T29-REDD1 cells displayed statistically significant increases in cell proliferation and colony formation compared with findings in parental T80 and T29 cells, whereas cells expressing REDD1 siRNA showed reduced cell proliferation and colony formation compared with parental cells infected with scrambled siRNA. (C) Cell proliferation rate following overexpression of REDD1 or siRNA knockdown for all three groups. (D) Number of colonies of anchorage-independent cell growth on soft agar in the presence of REDD1 overexpression or knockdown. (p < 0.05).
Phospho) Src, supplied by Epitomics corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega dual stop and grow luciferase assay system
Figure 1. Effect of <t>REDD1</t> on tumor cell growth in vitro. (A) REDD1 expression was detected by Western blotting in T80, T80K, T80H, T29, T29K and T29H cell lines. (B) Western blot analysis showed increased REDD1 expression after introduction of REDD1 cDNA into immortalized ovarian epithelial cell lines T80 and T29 and decreased REDD1 expression after REDD1 siRNA knockdown in RAS-transformed ovarian epithelial T29H cells. (C and D) T80-REDD1 and T29-REDD1 cells displayed statistically significant increases in cell proliferation and colony formation compared with findings in parental T80 and T29 cells, whereas cells expressing REDD1 siRNA showed reduced cell proliferation and colony formation compared with parental cells infected with scrambled siRNA. (C) Cell proliferation rate following overexpression of REDD1 or siRNA knockdown for all three groups. (D) Number of colonies of anchorage-independent cell growth on soft agar in the presence of REDD1 overexpression or knockdown. (p < 0.05).
Dual Stop And Grow Luciferase Assay System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc beta actin
Figure 1. Effect of <t>REDD1</t> on tumor cell growth in vitro. (A) REDD1 expression was detected by Western blotting in T80, T80K, T80H, T29, T29K and T29H cell lines. (B) Western blot analysis showed increased REDD1 expression after introduction of REDD1 cDNA into immortalized ovarian epithelial cell lines T80 and T29 and decreased REDD1 expression after REDD1 siRNA knockdown in RAS-transformed ovarian epithelial T29H cells. (C and D) T80-REDD1 and T29-REDD1 cells displayed statistically significant increases in cell proliferation and colony formation compared with findings in parental T80 and T29 cells, whereas cells expressing REDD1 siRNA showed reduced cell proliferation and colony formation compared with parental cells infected with scrambled siRNA. (C) Cell proliferation rate following overexpression of REDD1 or siRNA knockdown for all three groups. (D) Number of colonies of anchorage-independent cell growth on soft agar in the presence of REDD1 overexpression or knockdown. (p < 0.05).
Beta Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc ser236 s6 ribosomal protein
Figure 1. Effect of <t>REDD1</t> on tumor cell growth in vitro. (A) REDD1 expression was detected by Western blotting in T80, T80K, T80H, T29, T29K and T29H cell lines. (B) Western blot analysis showed increased REDD1 expression after introduction of REDD1 cDNA into immortalized ovarian epithelial cell lines T80 and T29 and decreased REDD1 expression after REDD1 siRNA knockdown in RAS-transformed ovarian epithelial T29H cells. (C and D) T80-REDD1 and T29-REDD1 cells displayed statistically significant increases in cell proliferation and colony formation compared with findings in parental T80 and T29 cells, whereas cells expressing REDD1 siRNA showed reduced cell proliferation and colony formation compared with parental cells infected with scrambled siRNA. (C) Cell proliferation rate following overexpression of REDD1 or siRNA knockdown for all three groups. (D) Number of colonies of anchorage-independent cell growth on soft agar in the presence of REDD1 overexpression or knockdown. (p < 0.05).
Ser236 S6 Ribosomal Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc rabbit polyclonal
Figure 1. Effect of <t>REDD1</t> on tumor cell growth in vitro. (A) REDD1 expression was detected by Western blotting in T80, T80K, T80H, T29, T29K and T29H cell lines. (B) Western blot analysis showed increased REDD1 expression after introduction of REDD1 cDNA into immortalized ovarian epithelial cell lines T80 and T29 and decreased REDD1 expression after REDD1 siRNA knockdown in RAS-transformed ovarian epithelial T29H cells. (C and D) T80-REDD1 and T29-REDD1 cells displayed statistically significant increases in cell proliferation and colony formation compared with findings in parental T80 and T29 cells, whereas cells expressing REDD1 siRNA showed reduced cell proliferation and colony formation compared with parental cells infected with scrambled siRNA. (C) Cell proliferation rate following overexpression of REDD1 or siRNA knockdown for all three groups. (D) Number of colonies of anchorage-independent cell growth on soft agar in the presence of REDD1 overexpression or knockdown. (p < 0.05).
Rabbit Polyclonal, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc caspase 3
Figure 1. Effect of <t>REDD1</t> on tumor cell growth in vitro. (A) REDD1 expression was detected by Western blotting in T80, T80K, T80H, T29, T29K and T29H cell lines. (B) Western blot analysis showed increased REDD1 expression after introduction of REDD1 cDNA into immortalized ovarian epithelial cell lines T80 and T29 and decreased REDD1 expression after REDD1 siRNA knockdown in RAS-transformed ovarian epithelial T29H cells. (C and D) T80-REDD1 and T29-REDD1 cells displayed statistically significant increases in cell proliferation and colony formation compared with findings in parental T80 and T29 cells, whereas cells expressing REDD1 siRNA showed reduced cell proliferation and colony formation compared with parental cells infected with scrambled siRNA. (C) Cell proliferation rate following overexpression of REDD1 or siRNA knockdown for all three groups. (D) Number of colonies of anchorage-independent cell growth on soft agar in the presence of REDD1 overexpression or knockdown. (p < 0.05).
Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: Cell Reports

Article Title: Oxidative Stress Triggers Selective tRNA Retrograde Transport in Human Cells during the Integrated Stress Response

doi: 10.1016/j.celrep.2019.02.077

Figure Lengend Snippet:

Article Snippet: Rabbit Antibody against REDD1/DDIT4 , Novus Biologicals , Cat# NBP1-77321SS; RRID: AB_11036185.

Techniques: Recombinant, Cell Isolation, Reverse Transcription, SYBR Green Assay, Isolation, Sequencing, Software

Figure 1. Effect of REDD1 on tumor cell growth in vitro. (A) REDD1 expression was detected by Western blotting in T80, T80K, T80H, T29, T29K and T29H cell lines. (B) Western blot analysis showed increased REDD1 expression after introduction of REDD1 cDNA into immortalized ovarian epithelial cell lines T80 and T29 and decreased REDD1 expression after REDD1 siRNA knockdown in RAS-transformed ovarian epithelial T29H cells. (C and D) T80-REDD1 and T29-REDD1 cells displayed statistically significant increases in cell proliferation and colony formation compared with findings in parental T80 and T29 cells, whereas cells expressing REDD1 siRNA showed reduced cell proliferation and colony formation compared with parental cells infected with scrambled siRNA. (C) Cell proliferation rate following overexpression of REDD1 or siRNA knockdown for all three groups. (D) Number of colonies of anchorage-independent cell growth on soft agar in the presence of REDD1 overexpression or knockdown. (p < 0.05).

Journal: Cell cycle (Georgetown, Tex.)

Article Title: REDD1 is required for RAS-mediated transformation of human ovarian epithelial cells.

doi: 10.4161/cc.8.5.7887

Figure Lengend Snippet: Figure 1. Effect of REDD1 on tumor cell growth in vitro. (A) REDD1 expression was detected by Western blotting in T80, T80K, T80H, T29, T29K and T29H cell lines. (B) Western blot analysis showed increased REDD1 expression after introduction of REDD1 cDNA into immortalized ovarian epithelial cell lines T80 and T29 and decreased REDD1 expression after REDD1 siRNA knockdown in RAS-transformed ovarian epithelial T29H cells. (C and D) T80-REDD1 and T29-REDD1 cells displayed statistically significant increases in cell proliferation and colony formation compared with findings in parental T80 and T29 cells, whereas cells expressing REDD1 siRNA showed reduced cell proliferation and colony formation compared with parental cells infected with scrambled siRNA. (C) Cell proliferation rate following overexpression of REDD1 or siRNA knockdown for all three groups. (D) Number of colonies of anchorage-independent cell growth on soft agar in the presence of REDD1 overexpression or knockdown. (p < 0.05).

Article Snippet: The following antibodies were used: 1:500 dilution for rabbit polyclonal REDD1 antibody (Proteintech Group, Inc., Chicago, IL), 1:500 dilution for mouse monoclonal Bcl-2 antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA), 1 μg/mlofmouse monoclonal Anti-Bcl-xL (Calbiochem, San Diego, CA), 1:2000 dilution of mouse monoclonal Bax antibody (Santa Cruz iotechnology, Inc.,), 2.5 μg/ml of rabbit polyclonal Bcl-xs antibody (Calbiochem), 1 μg/ml of mouse monoclonal Caspase-8 antibody (Biolegend, San Diego, CA), 1:2000 dilution of mouse monoclonal Caspase-9 antibody (Biolegend), 1:1000 dilution of mouse monoclonal Caspase-10 antibody (Biolegend), 1:1000 dilution of rabbit polyclonal Caspase-1 antibody (Cell Signaling Technology, Danvers, MA), and 1:1000 dilution of rabbit polyclonal fat-associated protein with death domain (FADD) antibody (Cell Signaling Technologies) followed by incubation with secondary antibodies NA931 anti-mouse immunoglobulin or NA9340 anti-rabbit immunoglobulin horseradish peroxidase-linked F(ab)2 fragment (Amersham Biosciences, UK Limited, Little Chalfont HP7 9NA, UK).

Techniques: In Vitro, Expressing, Western Blot, Knockdown, Transformation Assay, Infection, Over Expression

Figure 2. Effect of REDD1 on tumor growth in nude mice. (A and B) Tumor growth curve following s.c. injection of T80-REDD1 (p < 0.01, n = 4) and T29- REDD1 cells (p < 0.05, n = 4). (C) Tumor growth curve for s.c. tumors in nude mice inoculated with T29H and T29H-REDD11i cells (p < 0.01, n = 5). (D) Histopathologic analysis of xenograft tumors produced by i.p. injection of T29-REDD1 cells showed morphology of high-grade papillary serous carcinoma. Immunohistochemical positive staining of SV40, p53, CA125 and MUC2. (x400).

Journal: Cell cycle (Georgetown, Tex.)

Article Title: REDD1 is required for RAS-mediated transformation of human ovarian epithelial cells.

doi: 10.4161/cc.8.5.7887

Figure Lengend Snippet: Figure 2. Effect of REDD1 on tumor growth in nude mice. (A and B) Tumor growth curve following s.c. injection of T80-REDD1 (p < 0.01, n = 4) and T29- REDD1 cells (p < 0.05, n = 4). (C) Tumor growth curve for s.c. tumors in nude mice inoculated with T29H and T29H-REDD11i cells (p < 0.01, n = 5). (D) Histopathologic analysis of xenograft tumors produced by i.p. injection of T29-REDD1 cells showed morphology of high-grade papillary serous carcinoma. Immunohistochemical positive staining of SV40, p53, CA125 and MUC2. (x400).

Article Snippet: The following antibodies were used: 1:500 dilution for rabbit polyclonal REDD1 antibody (Proteintech Group, Inc., Chicago, IL), 1:500 dilution for mouse monoclonal Bcl-2 antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA), 1 μg/mlofmouse monoclonal Anti-Bcl-xL (Calbiochem, San Diego, CA), 1:2000 dilution of mouse monoclonal Bax antibody (Santa Cruz iotechnology, Inc.,), 2.5 μg/ml of rabbit polyclonal Bcl-xs antibody (Calbiochem), 1 μg/ml of mouse monoclonal Caspase-8 antibody (Biolegend, San Diego, CA), 1:2000 dilution of mouse monoclonal Caspase-9 antibody (Biolegend), 1:1000 dilution of mouse monoclonal Caspase-10 antibody (Biolegend), 1:1000 dilution of rabbit polyclonal Caspase-1 antibody (Cell Signaling Technology, Danvers, MA), and 1:1000 dilution of rabbit polyclonal fat-associated protein with death domain (FADD) antibody (Cell Signaling Technologies) followed by incubation with secondary antibodies NA931 anti-mouse immunoglobulin or NA9340 anti-rabbit immunoglobulin horseradish peroxidase-linked F(ab)2 fragment (Amersham Biosciences, UK Limited, Little Chalfont HP7 9NA, UK).

Techniques: Injection, Produced, Immunohistochemical staining, Staining

Figure 3. Level of apoptosis in ovarian cancer cells following REDD1 expression or knockdown. (A) Level of apoptosis as measured by flow cytometry. (B) Expression of key signaling molecules regulating the apoptotic pathway in T80-REDD1 and T29-REDD1 cells. (C) Model describing REDD1-mediated ovarian tumorigenesis. RAS activates expression of REDD1, which in turn inhibits apoptosis and promotes proliferation; both mechanisms synergistically promote ovarian malignant progression.

Journal: Cell cycle (Georgetown, Tex.)

Article Title: REDD1 is required for RAS-mediated transformation of human ovarian epithelial cells.

doi: 10.4161/cc.8.5.7887

Figure Lengend Snippet: Figure 3. Level of apoptosis in ovarian cancer cells following REDD1 expression or knockdown. (A) Level of apoptosis as measured by flow cytometry. (B) Expression of key signaling molecules regulating the apoptotic pathway in T80-REDD1 and T29-REDD1 cells. (C) Model describing REDD1-mediated ovarian tumorigenesis. RAS activates expression of REDD1, which in turn inhibits apoptosis and promotes proliferation; both mechanisms synergistically promote ovarian malignant progression.

Article Snippet: The following antibodies were used: 1:500 dilution for rabbit polyclonal REDD1 antibody (Proteintech Group, Inc., Chicago, IL), 1:500 dilution for mouse monoclonal Bcl-2 antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA), 1 μg/mlofmouse monoclonal Anti-Bcl-xL (Calbiochem, San Diego, CA), 1:2000 dilution of mouse monoclonal Bax antibody (Santa Cruz iotechnology, Inc.,), 2.5 μg/ml of rabbit polyclonal Bcl-xs antibody (Calbiochem), 1 μg/ml of mouse monoclonal Caspase-8 antibody (Biolegend, San Diego, CA), 1:2000 dilution of mouse monoclonal Caspase-9 antibody (Biolegend), 1:1000 dilution of mouse monoclonal Caspase-10 antibody (Biolegend), 1:1000 dilution of rabbit polyclonal Caspase-1 antibody (Cell Signaling Technology, Danvers, MA), and 1:1000 dilution of rabbit polyclonal fat-associated protein with death domain (FADD) antibody (Cell Signaling Technologies) followed by incubation with secondary antibodies NA931 anti-mouse immunoglobulin or NA9340 anti-rabbit immunoglobulin horseradish peroxidase-linked F(ab)2 fragment (Amersham Biosciences, UK Limited, Little Chalfont HP7 9NA, UK).

Techniques: Expressing, Knockdown, Flow Cytometry