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Image Search Results
Journal: Cell Reports
Article Title: Oxidative Stress Triggers Selective tRNA Retrograde Transport in Human Cells during the Integrated Stress Response
doi: 10.1016/j.celrep.2019.02.077
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Cell Isolation, Reverse Transcription, SYBR Green Assay, Isolation, Sequencing, Software
Journal: Cell cycle (Georgetown, Tex.)
Article Title: REDD1 is required for RAS-mediated transformation of human ovarian epithelial cells.
doi: 10.4161/cc.8.5.7887
Figure Lengend Snippet: Figure 1. Effect of REDD1 on tumor cell growth in vitro. (A) REDD1 expression was detected by Western blotting in T80, T80K, T80H, T29, T29K and T29H cell lines. (B) Western blot analysis showed increased REDD1 expression after introduction of REDD1 cDNA into immortalized ovarian epithelial cell lines T80 and T29 and decreased REDD1 expression after REDD1 siRNA knockdown in RAS-transformed ovarian epithelial T29H cells. (C and D) T80-REDD1 and T29-REDD1 cells displayed statistically significant increases in cell proliferation and colony formation compared with findings in parental T80 and T29 cells, whereas cells expressing REDD1 siRNA showed reduced cell proliferation and colony formation compared with parental cells infected with scrambled siRNA. (C) Cell proliferation rate following overexpression of REDD1 or siRNA knockdown for all three groups. (D) Number of colonies of anchorage-independent cell growth on soft agar in the presence of REDD1 overexpression or knockdown. (p < 0.05).
Article Snippet: The following antibodies were used: 1:500 dilution for
Techniques: In Vitro, Expressing, Western Blot, Knockdown, Transformation Assay, Infection, Over Expression
Journal: Cell cycle (Georgetown, Tex.)
Article Title: REDD1 is required for RAS-mediated transformation of human ovarian epithelial cells.
doi: 10.4161/cc.8.5.7887
Figure Lengend Snippet: Figure 2. Effect of REDD1 on tumor growth in nude mice. (A and B) Tumor growth curve following s.c. injection of T80-REDD1 (p < 0.01, n = 4) and T29- REDD1 cells (p < 0.05, n = 4). (C) Tumor growth curve for s.c. tumors in nude mice inoculated with T29H and T29H-REDD11i cells (p < 0.01, n = 5). (D) Histopathologic analysis of xenograft tumors produced by i.p. injection of T29-REDD1 cells showed morphology of high-grade papillary serous carcinoma. Immunohistochemical positive staining of SV40, p53, CA125 and MUC2. (x400).
Article Snippet: The following antibodies were used: 1:500 dilution for
Techniques: Injection, Produced, Immunohistochemical staining, Staining
Journal: Cell cycle (Georgetown, Tex.)
Article Title: REDD1 is required for RAS-mediated transformation of human ovarian epithelial cells.
doi: 10.4161/cc.8.5.7887
Figure Lengend Snippet: Figure 3. Level of apoptosis in ovarian cancer cells following REDD1 expression or knockdown. (A) Level of apoptosis as measured by flow cytometry. (B) Expression of key signaling molecules regulating the apoptotic pathway in T80-REDD1 and T29-REDD1 cells. (C) Model describing REDD1-mediated ovarian tumorigenesis. RAS activates expression of REDD1, which in turn inhibits apoptosis and promotes proliferation; both mechanisms synergistically promote ovarian malignant progression.
Article Snippet: The following antibodies were used: 1:500 dilution for
Techniques: Expressing, Knockdown, Flow Cytometry